Endocytosis and Membrane Turnover in the Germ Tube ofUromyces fabae
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Endocytosis and membrane turnover in the germ tube of uromyces fabae
We have used the fluorescent dye FM4-64 as a tracer to demonstrate bulk membrane internalization (endocytosis) and redistribution of the dye within the cytoplasm of the germ tube of the rust fungus Uromyces fabae. Staining of the hyphal membrane was detected 4 s after application of FM4-64 and reached a maximum after 1 min. The highest fluorescence intensity occurred in the apex. Subsequently, ...
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Using plasma membrane-enriched vesicles, the properties of the H+-ATPase (EC 3.6.1.35) from the rust fungus Uromyces viciae-fabae were studied. The enzyme is strictly Mg2+-dependent and is inhibited by vanadate. The pH-optimum is at 6.7. By Western blot analysis using a monoclonal antibody against corn plasma membrane H+-ATPase a polypeptide of approximately 104 kDa could be detected. The vanad...
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coli and has shown that it is able to trimerize into a triskelion " hub, " to bind light chains, and to assemble and Marino Zerial ‡ *Department of Clinical Biochemistry into a hexagonal lattice. Moreover, her lab has now crys-talized the recombinant clathrin, so detailed structural University of Cambridge Cambridge CB2 2QR information about assembly should be forthcoming. One of the hallmarks ...
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ژورنال
عنوان ژورنال: Fungal Genetics and Biology
سال: 1998
ISSN: 1087-1845
DOI: 10.1006/fgbi.1998.1059